Osteryoung square wave voltammetric determination of lactose in food samples by a derivative procedure

A method for determination of lactose in food samples by Osteryoung square wave voltammetry (OSWV) was developed. It was based on the nucleophilic addition reaction between lactose and aqua ammonia. The carbonyl group of lactose can be changed into imido group, and this increases the electrochemical...

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Bibliographic Details
Published in:Chinese chemical letters 2008-12, Vol.19 (12), p.1491-1494
Main Authors: Ni, Yong Nian, Wang, Yue Rong, Kokot, Serge
Format: Article
Language:English
Subjects:
Food
Lactose
Milk
Osteryoung square wave voltammetry
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Summary:A method for determination of lactose in food samples by Osteryoung square wave voltammetry (OSWV) was developed. It was based on the nucleophilic addition reaction between lactose and aqua ammonia. The carbonyl group of lactose can be changed into imido group, and this increases the electrochemical activity in reduction and the sensitivity. The optimal condition for the nucleophilic addition reaction was investigated and it was found that in NH 4Cl–NH 3 buffer of pH 10.1, the linear range between the peak current and the concentration of lactose was 0.6–8.4 mg L −1, and the detection limits was 0.44 mg L −1. The proposed method was applied to the determination of lactose in food samples and satisfactory results were obtained.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2008.09.052