Determination of levels of adenosine phosphates in blood by ion chromatography

A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and de...

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Bibliographic Details
Published in:Chinese chemical letters 2011-12, Vol.22 (12), p.1485-1488
Main Authors: Xie, Pei Jin, Ye, Ming Li, Hu, Zhong Yang, Pan, Guang Wen, Zhu, Yan, Zhang, Jia Jie
Format: Article
Language:English
Subjects:
Adenosine phosphate
ATP水平
Blood
Ion chromatography
IonPac
三磷酸腺苷
同时测定
电导检测器
离子色谱法
血液样本
阴离子交换色谱法
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Summary:A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2011.09.008