Application of differential pulse stripping voltammetry and chemometrics for the determination of three antibiotic drugs in food samples

• Published in: Chinese chemical letters 2012-03, Vol.23 (3), p.339-342
• Main Authors: Zhong, Yong Sheng, Ni, Yong Nian, Kokot, Serge
• Format: Article
• Language: English
• Subjects: Chemometrics; Differential pulse stripping voltammetry; Fluoroquinolone antibiotics; Food samples; PCR扩增; RBF神经网络; 化学计量学方法; 同时测定; 左氧氟沙星; 差分脉冲溶出伏安法; 应用; 抗生素药物
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2012.01.007

A reliable method for simultaneous determination of three antibiotic drugs（levofloxacin,gatifloxacin and lomefloxacin） by differential pulse stripping voltammetry（DPSV） in Britton-Robinson buffer（pH 7.96） was presented.The method is based on adsorptive accumulation of the antibacterial drugs on a hanging mercury dropping electrode（HMDE）,followed by the reduction of the adsorptive species by the technique of DPSV.Optimal conditions,the deposition time of 80 s,the deposition potential of—1250 mV,and the scan rate of 25 mV/s,were obtained.The linear concentration ranges of 0.010-0.080μg/mL were obtained for all these three antibiotic drugs,while the detection limits were 2.38,3.20 and 1.60ng/mL for levofloxacin,gatifloxacin and lomefloxacin,respectively.In this work,chemometrics methods,such as classical least squares（CLS）,partial least squares（PLS）, principle component regression（PCR） and radial basis function-artificial neural networks（RBF-ANN）,were used to quantitatively resolve the overlapping signals.It was found that PCR gave the best results with total relative prediction error（RPE_T） of 7.71%.The proposed method was applied to determine these three drugs in several commercial food samples with spiked method and yielded satisfactory recoveries.