Open tubular capillary electrochromatography with block co-polymer coating for separation of β-lactam antibiotics

[Display omitted] Unique block co-polymer P(MAn-St-NIPAm) has been successfully synthesized by reversible addition fragmentation chain transfer radical polymerization protocol. Based on the tunable hydrophobic/hydrophilic properties of the block co-polymer, a new open tubular capillary electrochroma...

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Bibliographic Details
Published in:Chinese chemical letters 2019-02, Vol.30 (2), p.349-352
Main Authors: Zhao, Liping, Qiao, Juan, Zhang, Hongyi, Xie, Fangnan, Qi, Li
Format: Article
Language:English
Subjects:
Block co-polymer coating
Open-tubular capillary Electrochromatography
Serum drugs separation
β-Lactam antibiotics
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Summary:[Display omitted] Unique block co-polymer P(MAn-St-NIPAm) has been successfully synthesized by reversible addition fragmentation chain transfer radical polymerization protocol. Based on the tunable hydrophobic/hydrophilic properties of the block co-polymer, a new open tubular capillary electrochromatography (OT-CEC) system has been constructed with the prepared block co-polymer as the coating and applied in analysis of β-lactam antibiotics in serum samples. A new open-tubular capillary electrochromatography (OT-CEC) method for analysis of β-lactam antibiotics has been developed with unique block co-polymer coating. To obtain the highly ordered block polymer chains, reversible addition fragmentation chain transfer radical polymerization method was used to synthesize poly(maleic anhydride-styrene-N-isopropylacrylamide). The prepared block co-polymer coating was characterized with NMR, fourier transform infrared spectroscopy and scanning electron microscope. Several key separation factors of OT-CEC, which including polymer amount, stability of the coating, temperature, species of organic additives, buffer pH and concentration, were investigated in detail. Our results indicated that the separation efficiency was improved greatly with the coating capillary and the three test analytes could be baseline separated. Then, the separation mechanism was briefly explored. Moreover, the proposed OT-CEC method displayed promising quantitative analysis property of the three test analytes with good linearity (R2>0.99), repeatability (relative standard deviations
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2018.03.007