A bright two-photon fluorescent probe for real-time monitoring autophagy in living cells

A highly bright two-photon fluorescent probe Lyso-OSC, was developed for highly sensitive real-time detection of autophagy process in living cells. [Display omitted] A novel donor-acceptor (D-A) type of two-photon (TP) fluorescent probe, i.e. Lyso-OSC, based on the lysosome-targeting morpholine grou...

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Bibliographic Details
Published in:Chinese chemical letters 2020-02, Vol.31 (2), p.447-450
Main Authors: Jiang, Chuankun, Li, Longchun, Jiang, Jiacheng, Hou, Lilin, Fang, Gemin, Haizhu, Yu, Meng, Xiangming
Format: Article
Language:English
Subjects:
Autophagy
Lysosome-targeting
Polarity
Real-time
Two-photon
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Summary:A highly bright two-photon fluorescent probe Lyso-OSC, was developed for highly sensitive real-time detection of autophagy process in living cells. [Display omitted] A novel donor-acceptor (D-A) type of two-photon (TP) fluorescent probe, i.e. Lyso-OSC, based on the lysosome-targeting morpholine group was developed. The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor. The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent. The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 μm, respectively. The strong fluorescence, high sensitivity to polarity, low cytotoxicity, and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes of cellular environment. To this end, a bright, real-time imaging autophagy of living cells has been achieved.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2019.07.046