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Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus
Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change pat...
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Published in: | Chinese journal of oceanology and limnology 2008-02, Vol.26 (1), p.62-68 |
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description | Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree. |
doi_str_mv | 10.1007/s00343-008-0062-z |
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The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.</description><identifier>ISSN: 0254-4059</identifier><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 1993-5005</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-008-0062-z</identifier><language>eng</language><publisher>Beijing: Science Press</publisher><subject>Animal embryos ; Antigens ; Aquaculture ; Biochemistry ; Cambaridae ; Cherax ; Cherax quadricarinatus ; Chymotrypsin ; Crayfish ; Crustaceans ; Developmental stages ; Earth and Environmental Science ; Earth Sciences ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; Enzymatic activity ; Enzyme activity ; Enzymes ; Freshwater ; Freshwater crustaceans ; Gene expression ; Hatching ; Larvae ; Marine biology ; Nucleotide sequence ; Oceanography ; Proteins ; Regulatory mechanisms (biology) ; Ribonucleic acid ; RNA ; Trypsin ; Trypsin gene ; 消化酶活性 ; 胰凝乳蛋白酶 ; 胰岛素</subject><ispartof>Chinese journal of oceanology and limnology, 2008-02, Vol.26 (1), p.62-68</ispartof><rights>Science Press 2008</rights><rights>Science Press 2008.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-7a7398c356cda1678087508c218008253c1e2cc6db67eebb811132e6d34510843</citedby><cites>FETCH-LOGICAL-c434t-7a7398c356cda1678087508c218008253c1e2cc6db67eebb811132e6d34510843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84119X/84119X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>罗文 赵云龙 周忠良 安传光 马强</creatorcontrib><title>Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus</title><title>Chinese journal of oceanology and limnology</title><addtitle>Chin. J. Ocean. Limnol</addtitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><description>Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.</description><subject>Animal embryos</subject><subject>Antigens</subject><subject>Aquaculture</subject><subject>Biochemistry</subject><subject>Cambaridae</subject><subject>Cherax</subject><subject>Cherax quadricarinatus</subject><subject>Chymotrypsin</subject><subject>Crayfish</subject><subject>Crustaceans</subject><subject>Developmental stages</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Freshwater</subject><subject>Freshwater crustaceans</subject><subject>Gene expression</subject><subject>Hatching</subject><subject>Larvae</subject><subject>Marine biology</subject><subject>Nucleotide sequence</subject><subject>Oceanography</subject><subject>Proteins</subject><subject>Regulatory mechanisms (biology)</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Trypsin</subject><subject>Trypsin gene</subject><subject>消化酶活性</subject><subject>胰凝乳蛋白酶</subject><subject>胰岛素</subject><issn>0254-4059</issn><issn>2096-5508</issn><issn>1993-5005</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkV9r1jAUxoso-Dr9AN4FLwTBupOkaZvL8fpng7HB0OuQpqdtZpu-b9Juaz-9GR0KXigkhMDveXjOeZLkLYVPFKA4DQA84ylAGW_O0vVZsqNS8lQAiOfJDpjI0gyEfJm8CuE20jIDuUu6z7bFMNk7JOjWZUCiTfzZaSHa1WS4uTojPd5hT8aGTH45BOtIPDhUfhmdNcRjbXp9T4zXS2ND95HsO_T6gRxnXXtrtHd6msPr5EWj-4Bvnt6T5MfXL9_35-nl9beL_dllajKeTWmhCy5Lw0Vuak3zooSyEFAaRss4GxPcUGTG5HWVF4hVVVJKOcO85pmgUGb8JPmw-d5r12jXqttxjgn6oNa2W7r1oWLRCOLSZGTfb-zBj8c5rkENNhjse-1wnIOisoCCiTyC7_4Cf7syyGSMKMT_IVbQCNENMn4MwWOjDt4O2i-KgnpsUm1NqhhSPTap1qhhmyZE1rXo_xj_S_SUxnSja49Rpyptfja2R8VyQSWXwH8BqK6rRg</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>罗文 赵云龙 周忠良 安传光 马强</creator><general>Science Press</general><general>Springer Nature B.V</general><general>School of Life Science,College of Shaoxing Arts and Sciences,Shaoxing 312000,China</general><general>School of Life Science,East China Normal University,Shanghai 200062,China%School of Life Science,East China Normal University,Shanghai 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level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus</title><author>罗文 赵云龙 周忠良 安传光 马强</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-7a7398c356cda1678087508c218008253c1e2cc6db67eebb811132e6d34510843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal embryos</topic><topic>Antigens</topic><topic>Aquaculture</topic><topic>Biochemistry</topic><topic>Cambaridae</topic><topic>Cherax</topic><topic>Cherax quadricarinatus</topic><topic>Chymotrypsin</topic><topic>Crayfish</topic><topic>Crustaceans</topic><topic>Developmental stages</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Embryogenesis</topic><topic>Embryonic development</topic><topic>Embryonic growth stage</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Freshwater</topic><topic>Freshwater crustaceans</topic><topic>Gene expression</topic><topic>Hatching</topic><topic>Larvae</topic><topic>Marine biology</topic><topic>Nucleotide sequence</topic><topic>Oceanography</topic><topic>Proteins</topic><topic>Regulatory mechanisms (biology)</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Trypsin</topic><topic>Trypsin gene</topic><topic>消化酶活性</topic><topic>胰凝乳蛋白酶</topic><topic>胰岛素</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>罗文 赵云龙 周忠良 安传光 马强</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology 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Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese journal of oceanology and limnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>罗文 赵云龙 周忠良 安传光 马强</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus</atitle><jtitle>Chinese journal of oceanology and limnology</jtitle><stitle>Chin. J. Ocean. Limnol</stitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>26</volume><issue>1</issue><spage>62</spage><epage>68</epage><pages>62-68</pages><issn>0254-4059</issn><issn>2096-5508</issn><eissn>1993-5005</eissn><eissn>2523-3521</eissn><abstract>Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.</abstract><cop>Beijing</cop><pub>Science Press</pub><doi>10.1007/s00343-008-0062-z</doi><tpages>7</tpages></addata></record> |
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subjects | Animal embryos Antigens Aquaculture Biochemistry Cambaridae Cherax Cherax quadricarinatus Chymotrypsin Crayfish Crustaceans Developmental stages Earth and Environmental Science Earth Sciences Embryogenesis Embryonic development Embryonic growth stage Enzymatic activity Enzyme activity Enzymes Freshwater Freshwater crustaceans Gene expression Hatching Larvae Marine biology Nucleotide sequence Oceanography Proteins Regulatory mechanisms (biology) Ribonucleic acid RNA Trypsin Trypsin gene 消化酶活性 胰凝乳蛋白酶 胰岛素 |
title | Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus |
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