Preliminarily functional analysis of a cloned novel human gene ADAM29

R3; ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was clone...

Full description

Saved in:
Bibliographic Details
Published in:中国科学C辑(英文版) 2001, Vol.44 (4), p.392-399
Main Authors: WANG Fang, XU Rener, ZHU Pengcheng, Hu Junjie, YING Beibei, ZHAO Shouyuan, LI Changben
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 399
container_issue 4
container_start_page 392
container_title 中国科学C辑(英文版)
container_volume 44
creator WANG Fang
XU Rener
ZHU Pengcheng
Hu Junjie
YING Beibei
ZHAO Shouyuan
LI Changben
description R3; ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.
doi_str_mv 10.3969/j.issn.1674-7305.2001.04.007
format article
fullrecord <record><control><sourceid>wanfang_jour</sourceid><recordid>TN_cdi_wanfang_journals_zgkx_ec200104007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>zgkx_ec200104007</wanfj_id><sourcerecordid>zgkx_ec200104007</sourcerecordid><originalsourceid>FETCH-wanfang_journals_zgkx_ec2001040073</originalsourceid><addsrcrecordid>eNqVjr0KwjAUhTMo-PsOd3Bwabz9sTWjaMVFcHAvoaY1Nd5AY9X69FbwBVzOgcPH4WNs5iMPRSwWFdfOEffjJPKSEJc8QPQ5Rhwx6bGhjxh7otsHbORchRiKZbQasvRYK6NvmmStTQtFQ_ldW5IGZBet0w5sARJyY0mdgexDGbg0N0lQKlKw3q4PgZiwfiGNU9Nfj9l8l542e-8pqZBUZpVt6u7PZe_y-spU_pXDqFML_0A_hB9GBA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Preliminarily functional analysis of a cloned novel human gene ADAM29</title><source>Springer Nature</source><creator>WANG Fang ; XU Rener ; ZHU Pengcheng ; Hu Junjie ; YING Beibei ; ZHAO Shouyuan ; LI Changben</creator><creatorcontrib>WANG Fang ; XU Rener ; ZHU Pengcheng ; Hu Junjie ; YING Beibei ; ZHAO Shouyuan ; LI Changben</creatorcontrib><description>R3; ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.</description><identifier>ISSN: 1006-9305</identifier><identifier>DOI: 10.3969/j.issn.1674-7305.2001.04.007</identifier><language>eng</language><publisher>School of Life Science, Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shang-hai 200433, China</publisher><ispartof>中国科学C辑(英文版), 2001, Vol.44 (4), p.392-399</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgkx-ec/zgkx-ec.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>WANG Fang</creatorcontrib><creatorcontrib>XU Rener</creatorcontrib><creatorcontrib>ZHU Pengcheng</creatorcontrib><creatorcontrib>Hu Junjie</creatorcontrib><creatorcontrib>YING Beibei</creatorcontrib><creatorcontrib>ZHAO Shouyuan</creatorcontrib><creatorcontrib>LI Changben</creatorcontrib><title>Preliminarily functional analysis of a cloned novel human gene ADAM29</title><title>中国科学C辑(英文版)</title><description>R3; ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.</description><issn>1006-9305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqVjr0KwjAUhTMo-PsOd3Bwabz9sTWjaMVFcHAvoaY1Nd5AY9X69FbwBVzOgcPH4WNs5iMPRSwWFdfOEffjJPKSEJc8QPQ5Rhwx6bGhjxh7otsHbORchRiKZbQasvRYK6NvmmStTQtFQ_ldW5IGZBet0w5sARJyY0mdgexDGbg0N0lQKlKw3q4PgZiwfiGNU9Nfj9l8l542e-8pqZBUZpVt6u7PZe_y-spU_pXDqFML_0A_hB9GBA</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>WANG Fang</creator><creator>XU Rener</creator><creator>ZHU Pengcheng</creator><creator>Hu Junjie</creator><creator>YING Beibei</creator><creator>ZHAO Shouyuan</creator><creator>LI Changben</creator><general>School of Life Science, Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shang-hai 200433, China</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2001</creationdate><title>Preliminarily functional analysis of a cloned novel human gene ADAM29</title><author>WANG Fang ; XU Rener ; ZHU Pengcheng ; Hu Junjie ; YING Beibei ; ZHAO Shouyuan ; LI Changben</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-wanfang_journals_zgkx_ec2001040073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG Fang</creatorcontrib><creatorcontrib>XU Rener</creatorcontrib><creatorcontrib>ZHU Pengcheng</creatorcontrib><creatorcontrib>Hu Junjie</creatorcontrib><creatorcontrib>YING Beibei</creatorcontrib><creatorcontrib>ZHAO Shouyuan</creatorcontrib><creatorcontrib>LI Changben</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>中国科学C辑(英文版)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG Fang</au><au>XU Rener</au><au>ZHU Pengcheng</au><au>Hu Junjie</au><au>YING Beibei</au><au>ZHAO Shouyuan</au><au>LI Changben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminarily functional analysis of a cloned novel human gene ADAM29</atitle><jtitle>中国科学C辑(英文版)</jtitle><date>2001</date><risdate>2001</risdate><volume>44</volume><issue>4</issue><spage>392</spage><epage>399</epage><pages>392-399</pages><issn>1006-9305</issn><abstract>R3; ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.</abstract><pub>School of Life Science, Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shang-hai 200433, China</pub><doi>10.3969/j.issn.1674-7305.2001.04.007</doi></addata></record>
fulltext fulltext
identifier ISSN: 1006-9305
ispartof 中国科学C辑(英文版), 2001, Vol.44 (4), p.392-399
issn 1006-9305
language eng
recordid cdi_wanfang_journals_zgkx_ec200104007
source Springer Nature
title Preliminarily functional analysis of a cloned novel human gene ADAM29
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T23%3A49%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preliminarily%20functional%20analysis%20of%20a%20cloned%20novel%20human%20gene%20ADAM29&rft.jtitle=%E4%B8%AD%E5%9B%BD%E7%A7%91%E5%AD%A6C%E8%BE%91(%E8%8B%B1%E6%96%87%E7%89%88)&rft.au=WANG%20Fang&rft.date=2001&rft.volume=44&rft.issue=4&rft.spage=392&rft.epage=399&rft.pages=392-399&rft.issn=1006-9305&rft_id=info:doi/10.3969/j.issn.1674-7305.2001.04.007&rft_dat=%3Cwanfang_jour%3Ezgkx_ec200104007%3C/wanfang_jour%3E%3Cgrp_id%3Ecdi_FETCH-wanfang_journals_zgkx_ec2001040073%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_wanfj_id=zgkx_ec200104007&rfr_iscdi=true