Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle

The present study examined the effects of genetic manipulation to the donor cell and different types of transgenic donor cells on developmental potential of bovine nuclear transfer (NT) embryos. Four types of bovine somatic cells, including granulosa cells, fetal fibroblasts, fetal oviduct epithelia...

Full description

Saved in:
Bibliographic Details
Published in:Science China. Life sciences 2004-04, Vol.47 (2), p.183-189
Main Authors: Gong, Guochun, Dai, Yunping, Fan, Baoliang, Zhu, Huabing, Wang, Haiping, Wang, Lili, Fang, Changge, Wan, Rong, Liu, Ying, Li, Rong, Li, Ning
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Blastocyst - physiology
Cattle
Cell lines
Developmental stages
Electroporation
Embryos
Epithelial cells
Female
Fetuses
Fibroblasts
Genes, Reporter
Granulosa cells
Granulosa Cells - cytology
Green fluorescent protein
Neomycin
Nuclear transfer
Nuclear Transfer Techniques
Oocytes - cytology
Oocytes - physiology
Ovaries
Oviduct
Restriction Mapping
Somatic cells
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study examined the effects of genetic manipulation to the donor cell and different types of transgenic donor cells on developmental potential of bovine nuclear transfer (NT) embryos. Four types of bovine somatic cells, including granulosa cells, fetal fibroblasts, fetal oviduct epithelial cells and fetal ovary epithelial cells, were transfected with a plasmid (pCE-EGFP-Ires-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant (Neor) genes by electroporation. After 14 days selection with 800 microg/mL G418, transgenic cell lines from each type of somatic cells were obtained. Nontransgenic granulosa cells and all 4 types of transgenic somatic cells were used as nuclear donor to produce transgenic embryos by NT. There was no significant difference in development rates to the blastocyst stage for NT embryos from transgenic and nontransgenic granulosa cells (44.6% and 42.8%, respectively), and transfer of NT embryos derived from transgenic and nontransgenic granulosa cells to recipients resulted in similar pregnancy rates on day 90 (19% and 25%, respectively). The development rates to the blastocyst stage of NT embryos were significantly different among different types of transgenic donor cells (P
ISSN:1006-9305
1674-7305
1869-1889
DOI:10.1360/03yc0015