Applications of the double-barreled data in whole-genome shotgun sequence assembly and analysis

Double-barreled (DB) data have been widely used for the assembly of large genomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly procedure and report on novel applications...

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Bibliographic Details
Published in:Science China. Life sciences 2005-06, Vol.48 (3), p.300-306
Main Authors: Han, Yujun, Ni, Peixiang, Lü, Hong, Ye, Jia, Hu, Jianfei, Chen, Chen, Huang, Xiangang, Cong, Lijuan, Li, Guangyuan, Wang, Jing, Gu, Xiaocheng, Yu, Jun, Li, Songgang
Format: Article
Language:English
Subjects:
Cloning, Molecular - methods
DNA, Plant - chemistry
DNA, Plant - genetics
Gene Library
Genome, Plant
Reproducibility of Results
Sequence Analysis, DNA - methods
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Summary:Double-barreled (DB) data have been widely used for the assembly of large genomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly procedure and report on novel applications during the following data-mining processes such as acquiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/BF03183625