Identification of prior candidate genes for Sclerotinia local resistance in Brassica napus using Arabidopsis cDNA microarray and Brassica-Arabidopsis comparative mapping

Arabidopsis cDNA arrays were used to screen the local-defense-associated genes in oilseed rape (Brassica napus L.) at the challenge of Sclerotinia sclerotiorum. 61 genes with two-fold expression changes were screened out from the local tissue around the necrosis. Among them, 36 unique genes were up-...

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Bibliographic Details
Published in:Science China. Life sciences 2005-10, Vol.48 (5), p.460-470
Main Authors: Liu, Renhu, Zhao, Jianwei, Xiao, Yong, Meng, Jinling
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - microbiology
Ascomycota - pathogenicity
Brassica
Brassica napus
Brassica napus - genetics
Brassica napus - microbiology
DNA microarrays
Gene Expression Profiling
Gene mapping
Genes, Plant
Oligonucleotide Array Sequence Analysis
Physical Chromosome Mapping
Plant Diseases - genetics
Plant Diseases - microbiology
Quantitative Trait Loci - genetics
Sclerotinia
Sclerotinia sclerotiorum
Sequence Homology, Nucleic Acid
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Summary:Arabidopsis cDNA arrays were used to screen the local-defense-associated genes in oilseed rape (Brassica napus L.) at the challenge of Sclerotinia sclerotiorum. 61 genes with two-fold expression changes were screened out from the local tissue around the necrosis. Among them, 36 unique genes were up-regulated and 25 unique genes were down-regulated. RT-PCR and Northern blot results were consistent with the array results, suggesting Arabidopsis arrays were useful for transcriptional profiling of B. napus genes. Some of these genes were located in the interval of some QTLs for Sclerotinia resistance in B. napus by Brassica-Arabidopsis comparative mapping. These genes may have priority to be pursued for more intensive research.
ISSN:1006-9305
1674-7305
1869-1889
DOI:10.1360/02yc0106