Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7

• Published in: Science China. Life sciences 2005, Vol.48 (5), p.506-514
• Main Authors: Li, Han, Zeng, Qunli, Weng, Yu, Lu, Deqiang, Jiang, Huai, Xu, Zhengping
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinogenicity; Carcinogens; Cell Line, Tumor; Chromatography, Liquid; Electromagnetic fields; Electromagnetic Fields - adverse effects; Electrophoresis, Gel, Two-Dimensional; Female; Gene Expression Profiling; Humans; Low frequency; Magnetic fields; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Peptide Fragments - biosynthesis; Peptide Fragments - genetics; Proteasomes; Protein expression; Proteins; Proteomics; RNA-binding protein; RNA-Binding Proteins - biosynthesis; RNA-Binding Proteins - genetics; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
• ISSN: 1006-9305; 1674-7305; 1869-1889
• DOI: 10.1360/062004-39

Extremely Low Frequency Magnetic Fields (ELF MF) has been considered as a "possible human carcinogen" by International Agency for Research on Cancer (IARC) while credible mechanisms of its carcinogenicity remain unknown. In this study, a proteomics approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell line MCF7, in order to determine ELF MF-responsive proteins. MCF7 cells were exposed to 50 Hz, 0.4 mT ELF MF for 24 h and the changes of protein profile were examined using two dimensional electrophoresis. Up to 6 spots have been statistically significantly altered (their expression levels were changed at least 5 fold up or down) compared with sham-exposed group. 19 ones were only detected in exposure group while 19 ones were missing. Three proteins were identified by LC-IT Tandem MS as RNA binding protein regulatory subunit, Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein. Our finding showed that 50 Hz, 0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological functions of normal cell and 2-DE coupled with MS is a promising approach to elucidating cellular effects of electromagnetic fields.