Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance

DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) 'Fall Color' genome. Compared with wild type plants, severe grow...

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Published in:Science China. Life sciences 2006-10, Vol.49 (5), p.436-445
Main Authors: Hong, Bo, Tong, Zheng, Ma, Nan, Li, Jianke, Kasuga, Mie, Yamaguchi-Shinozaki, Kazuko, Gao, Junping
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Chrysanthemum - drug effects
Chrysanthemum - genetics
Chrysanthemum - growth & development
Chrysanthemum - metabolism
Desiccation
Disasters
Electrolytes
Gene Expression - genetics
Phenotype
Plants, Genetically Modified
Seedlings - genetics
Seedlings - growth & development
Seedlings - metabolism
Sodium Chloride - pharmacology
Transcription Factors - genetics
Transcription Factors - metabolism
Water
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Summary:DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) 'Fall Color' genome. Compared with wild type plants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants. RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. The transgenic plants exhibited tolerance to drought and salt stress, and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants. Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions. These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum, and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/s11427-006-2014-1