A rhizobia strain isolated from root nodule of gymnosperm Podocarpus macrophyllus

Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macrophyllus and rhizobia has never been reported. In this study, 11 endophytic bacteria strains were isolated f...

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Bibliographic Details
Published in:Science China. Life sciences 2007-04, Vol.50 (2), p.228-233
Main Authors: Huang, BaoLing, Lü, ChengQun, Wu, Bo, Fan, LiQin
Format: Article
Language:English
Subjects:
Bacterial Proteins - metabolism
Base Sequence
Coniferophyta - microbiology
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Molecular Sequence Data
Nitrogen Fixation
Nitrogenase - metabolism
Phylogeny
Plant Roots - microbiology
Rhizobium - classification
Rhizobium - enzymology
Rhizobium - genetics
Rhizobium - isolation & purification
RNA, Ribosomal, 16S - genetics
Seedlings - microbiology
Symbiosis
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Summary:Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macrophyllus and rhizobia has never been reported. In this study, 11 endophytic bacteria strains were isolated from root nodules of P. macrophyllus and its variation P. macrophyllus var. maki. The plant infection tests on these strains indicated that the isolated strains could be nodulated on P. macrophyllus plants, and weak nitrogenase activity of nodules was found in acetylene reduction method. According to the physiological and biochemical characteristics of the 11 strains, GXLO 02 was selected as the representative strain. 16S rDNA full-length sequence analysis of GXLO 02 confirmed that the representative strain GXLO 02 belongs to Rhizobium sp.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/s11427-007-0034-0