Molecular characterization and developmental expression of the gene encoding the prothoracicotropic hormone in the beet armyworm, Spodoptera exigua

Prothoracicotropic hormone (PTTH), a neuropeptide hormone stimulating the prothoracic glands to synthesize ecdysone, plays an important role in regulating postembryonic development in insects. The cDNA encoding PTTH was isolated and sequenced from the beet armyworm, Spodoptera exigua (Spe). The dedu...

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Bibliographic Details
Published in:Science China. Life sciences 2007-08, Vol.50 (4), p.466-472
Main Authors: Xu, Jun, Su, JianYa, Shen, JinLiang, Xu, WeiHua
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary - genetics
Female
Gene Expression Regulation, Developmental
Genes, Insect
Helicoverpa armigera
Insect Hormones - genetics
Insect Hormones - metabolism
Lepidoptera
Male
Molecular Sequence Data
Phylogeny
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Spodoptera - genetics
Spodoptera - growth & development
Spodoptera - metabolism
Spodoptera exigua
Tissue Distribution
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Summary:Prothoracicotropic hormone (PTTH), a neuropeptide hormone stimulating the prothoracic glands to synthesize ecdysone, plays an important role in regulating postembryonic development in insects. The cDNA encoding PTTH was isolated and sequenced from the beet armyworm, Spodoptera exigua (Spe). The deduced amino acid sequence is composed of a signal peptide, a peptide (65 amino acids) of unknown function, and a mature PTTH molecule (111 amino acids). The Spe-PTTH shows similarities (45.5%-70.3%) to other known PTTHs reported in Lepidoptera species, but 7 cysteine residues and the hydrophobic regions were conserved. Whole-mount immunocytochemistry by using an antiserum against recombinant Helicoverpa armigera PTTH showed that Spe-PTTH was synthesized in two pairs of neurosecretory cells in the S. exigua brain. Northern blot analysis demonstrates the presence of a 1.2-kb transcript in the brain. The Spe-PTTH mRNA is detectable at high levels at the wandering larval stage, early pupal stage, and pharate adult stage, suggesting that the Spe-PTTH gene might be correlated with molting, metamorphosis, and reproduction.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/s11427-007-0060-y