Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures

• Published in: Agricultural sciences in China 2011-06, Vol.10 (6), p.813-819
• Main Authors: LIN, Xing-e, JI, Hong-qing, NIU, Jun-hai, HU, Yan-min, FU, Zhong-jun, LIU, Zong-hua, TANG, Ji-hua
• Format: Article
• Language: English
• Subjects: calcium; CIPK gene; cloning; complementary DNA; corn; expression; genes; maize; nucleotides; open reading frames; PK基因; pollen; pollen tubes; protein kinases; quantitative polymerase chain reaction; second messengers; suppression subtractive hybridization; temperature; 克隆; 抑制性消减杂交; 温度; 温敏核; 玉米; 自交不亲和性; 钙调神经磷酸酶
• ISSN: 1671-2927
• DOI: 10.1016/S1671-2927(11)60066-7

A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.