Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis

• Published in: Agricultural sciences in China 2011-07, Vol.10 (7), p.1106-1116
• Main Authors: TONG, Hui-li, GAO, Xue-jun, LI, Qing-zhang, LIU, Jie, LI, Nan, WAN, Zhong-ying
• Format: Article
• Language: English
• Subjects: adenosinetriphosphatase; AMPK; beta-casein; dairy cow; dairy cows; DBP; dibutyl phthalate; energy; energy metabolism; epithelial cells; fluorescent antibody technique; genes; glucose; lactation; mammary gland epithelial cell; mammary glands; metabolic regulation; milk fat; PCR检测; quantitative polymerase chain reaction; signal transduction; STAT5; Vaccaria hispanica subsp. hispanica; Vaccaria segetalis; Western blotting; 乳腺上皮细胞; 代谢调控; 分离; 复合; 奶牛
• ISSN: 1671-2927
• DOI: 10.1016/S1671-2927(11)60100-4

In previous experiment, we isolated a compound dibutyl phthalate （DBP） from Vaccaria segetalis which had galactopoietic function on mammary gland epithelial cells of dairy cow （DCMECs）. In this experiment, we ascertained the metabolic regulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, b-casein, Glut1, SREBP-1, PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot and immunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 and p-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates b-casein synthesis. DBP also raises the activities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPK downstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, the activities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolism level of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs.