Understanding the metabolism of Mycoplasma mycoides subsp.capri in vitro by a transcriptomic analysis

It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium.This brings a lot of difficulties to mycoplasma research and application,including Mycoplasma mycoides subsp.capri(Mmc).Furthermore,little research on the characteristi...

Full description

Saved in:
Bibliographic Details
Published in:农业科学学报（英文） 2018, Vol.17 (2), p.428-435
Main Authors: WANG Xiao-hui, WANG Yan-fang, HUANG Hai-bi, BAI Fan, SHI Xiao-na, MA Chang-jiao, GAO Yuan, ZHANG Jian-hua, ZHANG Wen-guang, HAO Yong-qing
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium.This brings a lot of difficulties to mycoplasma research and application,including Mycoplasma mycoides subsp.capri(Mmc).Furthermore,little research on the characteristics of Mmc metabolism has been reported.In this study,Mmc PG3 strain cultures were investigated for dynamic gene expression.Culture samples were harvested during logarithmic phase (PG3-1),stationary phase (PG3-2),decline phase (PG3-3) and late decline phase (PG3-4).Twelve RNA samples (three replicates for each of the four growth stages considered) from these cultures were collected and sequenced.Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes (P
ISSN:2095-3119
DOI:10.1016/S2095-3119(17)61747-7