Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice

• Published in: Rice science 2007-06, Vol.14 (2), p.85-93
• Main Authors: WAN, Ying-xiu, DENG, Qi-ming, WANG, Shi-quan, LIU, Ming-wei, ZHOU, Hua-qiang, LI, Ping
• Format: Article
• Language: English
• Subjects: amylose content; cleaved amplified polymorphic sequence; gel consistency; gelatinization temperature; rice; simple sequence repeat; single nucleotide polymorphism; waxy gene
• ISSN: 1672-6308; 1876-4762
• DOI: 10.1016/S1672-6308(07)60013-4

The allelic variation of the Wx gene in 50 non-glutinous rice varieties (lines) was analyzed by using the microsatellite marker RM190 [for (CT) n simple sequence repeat (SSR)] and cleaved amplified polymorphic sequence(CAPS) marker 484/W2R- ACC?[for G/T single nucleotide polymorphism (SNP)]. Six homozygous (CT) n types, namely (CT) 20, (CT) 19, (CT) 18, (CT) 17, (CT) 16, (CT) 14, (CT) 11 and (CT) 10, and a heterozygous genotype (CT) 11/(CT) 18 were detected for RM190, of which (CT) 11 and (CT) 18 were predominant. Two homozygous Wx genotypes (G/G and T/T) and one heterozygous (G/T) were detected using 484/W2R- ACC?. Most of the materials with a RM190 of (CT) 11 were G/G for SNP of 484/W2R- ACC I, while T/T for SNP was predominantly appeared in materials with (CT) 18. The materials tested could be grouped into 10 categories using the two markers together. Results indicated that 59.3% variance of amylose content was attributed to the polymorphism of Wx gene revealed by RM190, while 56.1% and 24.6% of the variances in amylose content and gel consistency were respectively to the polymorphism of Wx gene revealed by 484/W2R- ACC I. Furthermore, with both SSR and CAPS markers, 72.4% of the variance in amylose content could be explained. In addition, the application prospects of the two markers in breeding were also discussed.