A Rice CaMBP Gene is Induced in Organ-Specific Manner by Both Chilling and Heat-Shock Treatments

A rice CaMBP gene, OsCaMBP （AB363406）, was isolated from a chilling treated rice using the fluorescent differential display （FDD） screening method. Its cDNA sequence （2094 bp） contains an opening reading frame （ORF） encoding a 569 amino acids protein （63.2 kD）. OsCaMBP has the typical structural fea...

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Bibliographic Details
Published in:Rice science 2008-09, Vol.15 (3), p.166-172
Main Authors: WAN, Jia, XI, Jiang, DU, Zhi-ru, XU, Zheng-jun
Format: Article
Language:English
Subjects:
calmodulin-binding protein
CaMBP
fluorescent differential display
gene cloning
organ-specific expression
rice ( Oryza sativa)
基因克隆
水稻
遗传基因
钙调蛋白
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Summary:A rice CaMBP gene, OsCaMBP （AB363406）, was isolated from a chilling treated rice using the fluorescent differential display （FDD） screening method. Its cDNA sequence （2094 bp） contains an opening reading frame （ORF） encoding a 569 amino acids protein （63.2 kD）. OsCaMBP has the typical structural features of the CaMBP family, including the conserved IQ calmodulin-binding motif at the N-terminus. Homology analysis revealed 38.25%-47.28% identities of OsCaMBP with other CaMBPs in plants. RT-PCR analysis showed that the expression of OsCaMBP was remarkably inducible under the chilling （8℃） and heat-shock （42℃） treatments. OsCaMBP was undetectable under the normal conditions, and induced under the chilling treatment for 1 h, as well as the heat-shock treatment for 15 min, suggesting that the gene plays important roles in the signaling pathway in rice under both chilling and heat-shock stresses.
ISSN:1672-6308
1876-4762
DOI:10.1016/S1672-6308(08)60038-4