Fine Mapping of QTLs for Stigma Exsertion Rate from Oryza glaberrima by Chromosome Segment Substitution

• Published in: Rice science 2022-01, Vol.29 (1), p.55-66
• Main Authors: Quanya, Tan, Haitao, Zhu, Hui, Liu, Yuerong, Ni, Shengze, Wu, Xin, Luan, Junwei, Liu, Weifeng, Yang, Zifeng, Yang, Ruizhen, Zeng, Guifu, Liu, Shaokui, Wang, Guiquan, Zhang
• Format: Article
• Language: English
• Subjects: Oryza glaberrima; QTL; Single-segment substitution line; Stigma exsertion; Substitution mapping
• ISSN: 1672-6308; 1876-4762
• DOI: 10.1016/j.rsci.2021.12.005

Stigma exsertion is an important trait for outcrossing ability in rice. Stigma exsertion rate (SER) of male sterile lines (MSLs) is a key factor affecting F1-seed production in hybrid rice. In this study, seven QTLs for SER were detected on five chromosomes using a set of single-segment substitution lines (SSSLs) derived from O. glaberrima. Three of the QTLs were mapped in the estimated intervals of 92.5–333.0kb. qSER-5 was located in a substitution segment of 92.5kb. qSER-1b and qSER-8b were respectively limited to 333.0kb and 107.5kb by secondary substitution mapping. qSER-1b and qSER-3 had bigger additive effects of 11.5% and 11.9%, respectively, while the other five QTLs had smaller additive effects from 5.7% to 8.6%. Open reading frames were identified in the regions of qSER-5 and qSER-8b in O. sativa and O. glaberrima genomes. Fine mapping of the QTLs laid a foundation for the cloning of genes, and QTLs for SER will be used to develop MSLs with strong ability of outcrossing.