Effect of ephrin-B2 on the expressions of angiopoietin-1 and -2 atfer focal cerebral ischemia/reperfusion

Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intr...

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Published in:中国神经再生研究（英文版） 2016, Vol.11 (11), p.1784-1789
Main Authors: Hui Xiao, Qing Huang, Jia-qi Wang, Qing-qing Deng, Wen-ping Gu
Format: Article
Language:English
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Summary:Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 (Ang-1) mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 atfer reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 (Ang-2) were highly up-regulated at all time points tested. Double immunolfuorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. These ifndings indicate that ephrin-B2 inlfuences the expres-sions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.
ISSN:1673-5374
DOI:10.4103/1673-5374.194723