Establishment of submandibular gland allotransplantation model in miniature swine

Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substitute. However, severe xerophthalmia can be accompanied by salivary gland dysfunction,...

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Bibliographic Details
Published in:Chinese medical journal 2006-03, Vol.119 (6), p.482-487
Main Authors: Ge, Xi-yuan, Yu, Guang-yan, Cai, Zhi-gang, Mao, Chi
Format: Article
Language:English
Subjects:
Animals
Microscopy, Electron
Models, Animal
Submandibular Gland - pathology
Submandibular Gland - transplantation
Submandibular Gland - ultrastructure
Swine
Swine, Miniature
Transplantation, Homologous
下颌腺
动物模型
异体移植
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Summary:Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substitute. However, severe xerophthalmia can be accompanied by salivary gland dysfunction, making such autotransplantation unsuitable. Therefore, SMG allotransplantation might be a solution. The aim of this study was to assess the technical feasibility of submandibular gland allotransplantation. Methods Twelve miniature swine were randomized to serve as donors or recipients. One SMG was transplanted between a donor and a recipient. The donor SMG was revascularized by microvascular anastomosis of its vascular pedicle to the recipient lingual artery and external jugular vein. The secretory duct was implanted into the vestibule of the mouth through a subcutaneous tunnel. No ilnmunosuppressive agent was administered. The results were assessed by visual inspection of the secretion, and histopathological examination of the transplanted SMG. Results Technically, all surgical procedures were successful. Clear secretion flowed out of the duct as soon as blood supply of the transplanted submandibular gland was reestablished. The secretion of the gland lasted for 5 days. As expected, an acute rejection reaction occurred after surgery because no immunosuppressive agents were used. Secretion from the transplanted SMG ceased within 5 days. Conclusions A model of SMG allotransplantation can be established in miniature swine. The technique of submandibular gland allotransplantation is feasible.
ISSN:0366-6999
2542-5641
DOI:10.1097/00029330-200603020-00009