Chemokine SR-PSOX/CXCL16 expression in peripheral blood of patients with acute coronary syndrome

• Published in: Chinese medical journal 2008-01, Vol.121 (2), p.112-117
• Main Authors: Yang, Hui-ling, Xu, Yang-yan, DU, Li-fen, Liu, Chang-hui, Zhao, Qiang, Wei, Wu-jie, You, Yong, Quan, Zhi-hua
• Format: Article
• Language: English
• Subjects: Acute Coronary Syndrome - immunology; Blotting, Western; Chemokine CXCL16; Chemokines, CXC - blood; Chemokines, CXC - genetics; Coronary Angiography; Fluorescent Antibody Technique; Humans; Receptors, Scavenger - blood; Receptors, Scavenger - genetics; RNA, Messenger - blood; SR-PSOX/CXCL16; 单核细胞; 基因表达; 急性冠心病; 清除剂; 磷脂酰丝氨酸
• ISSN: 0366-6999; 2542-5641
• DOI: 10.1097/00029330-200801020-00004

Background Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 （SR-PSOX/CXCL16） promotes foam cell formation through the tumor necrosis factor （TNF）-α mediated mechanism. Because chemokine CXCL16 could be expressed in atherosclerotic lesions and induce smooth muscle cell （SMC） proliferation, we＇presume that the monocyte SR-PSOX/CXCL16 detection in the patients＇ peripheral blood will be important for early diagnosis and prognosis of atherosclerosis （AS）. Methods Enrolled in this study were 40 patients with acute coronary syndrome （ACS）, including 20 patients with acute myocardial infarction （AMI） and 20 patients with unstable angina pectoris （UAP）, and 20 normal controls. Monocytes in the peripheral blood were isolated, and the changes of expression of CXCL16/SR-PSOX mRNA were compared using reverse transcription-polymerase chain reaction （RT-PCR）, with β-actin as internal control. We compared the expression of CXCL16/SR-PSOX in the ACS subgroups, using Western-blot to analyze protein expression levels. Tissue sections were made from biopsy specimens taken from patients with infective endocarditis, liver cirrhosis, and lung cancer as well as normal controls. And the expression of CXCL16/SR- PSOX was analyzed with a confocal microscope. Results The expression of CXCL16/SR-PSOX mRNA and protein in the monocytes of peripheral blood was significantly higher in ACS patients than in normal controls （P〈0.05）; however, there was no significant difference in CXCL16/SR-PSOX expression between UAP group and AMI group （P〉0.05）. Immunofluorescence showed that there were low expression of SR-PSOX in normal vascular endothelial cells and enhanced expression in every layer of the infected vessels, while spreading from endothelial cells to surrounding tissues as infection worsens. Confocal microscopy showed that the expression of SR-PSOX was enhanced in the infiltrated lymphocytes in liver cirrhosis, and that the expression level was proportionate to the degree of inflammation in the portal hepatis and folia. Conclusions The expression of CXCL16/SR-PSOX in the monocytes of peripheral blood was significantly higher in ACS patients than in the controls. CXCL16/SR-PSOX-mediated inflammation may contribute to the pathogenesis of ACS, and CXCL16 may play an important role in the pathogenesis and development of AS in humans.