Reconstruction of orbital defect in rabbits with composite of calcium phosphate cement and recombinant human bone morphogenetic protein-2

• Published in: Chinese medical journal 2010-12, Vol.123 (24), p.3658-3662
• Main Authors: Zheng, Yong-Xin, Wang, Jing, Lin, Hao-Tian, Li, Ling
• Format: Article
• Language: English
• Subjects: Animals; Bone Cements - therapeutic use; Bone Morphogenetic Protein 2 - therapeutic use; Bone Substitutes - therapeutic use; Calcium Phosphates - therapeutic use; Female; Male; Microscopy, Electron, Scanning; Orbit - pathology; Orbit - surgery; Orbit - ultrastructure; Osteogenesis; Rabbits; Recombinant Proteins - therapeutic use; Reconstructive Surgical Procedures - methods; Transforming Growth Factor beta - therapeutic use; 复合材料; 扫描电子显微镜; 磷酸钙骨水泥; 组织学检查; 骨形态发生蛋白-2; 骨缺损
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.2010.24.027

Background Calcium phosphate cement （CPC） is a biocompatible and osteoconductive bone substitute, and recombinant human bone morphogenetic protein-2 （rhBMP-2） has strong osteoinductibility, therefore we developed a composite bone substitute with CPC and rhBMP-2 and evaluate its reconstruction effect in rabbit orbital defect.Methods Thirty-six rabbits were randomly divided into two groups and a 5 mmx5 mmx2 mm bone defect in the infraorbital rim was induced by surgery in each orbit （72 orbits in all）. The orbital defects were treated with pure CPC or composite of CPC and rhBMP-2. The osteogenesis ability of different bone substitute was evaluated by gross observation, histological examination, histomorphometrical evaluation, compressive load-to-failure testing, and scanning electron microscope （SEM）.Results Gross observation showed that both bone substitutes were safe and effective for reconstruction of orbital defect. However, histological examination, histomorphometrical evaluation and SEM showed that CPC/rhBMP-2 group had faster speed in new bone formation and degradation of substitute material than CPC group. Compressive load-to-failure testing showed that CPC/rhBMP-2 group had stronger compressive strength than CPC group at every stage with significant difference （P ＜0.05）.Conclusion Composite of CPC/rhBMP-2 is an ideal bioactive material for repairing orbital defect, with good osteoconductibility and osteoinductibility.