Effects of ovarian cancer G protein coupled receptor 1 on the proliferation, migration, and adhesion of human ovarian cancer cells

Background OGR1 was found as a G-protein coupled receptor （GPCR） and proton sensor. Our previous studies have found that OGR1 has inhibitory effect on the metastasis of prostate cancer. In order to investigate the roles of OGR1 gene in the biological activities of ovarian cancer, we studied the OGR1...

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Bibliographic Details
Published in:Chinese medical journal 2011-05, Vol.124 (9), p.1327-1332
Main Authors: Ren, Juan, Zhang, Long
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Adhesion - genetics
Cell Adhesion - physiology
Cell Line, Tumor
Cell Movement - genetics
Cell Movement - physiology
Cell Proliferation
G蛋白偶联受体
Humans
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Transfection
人类
卵巢癌细胞
基因转染
粘连
细胞增殖
细胞外基质
细胞迁移
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Summary:Background OGR1 was found as a G-protein coupled receptor （GPCR） and proton sensor. Our previous studies have found that OGR1 has inhibitory effect on the metastasis of prostate cancer. In order to investigate the roles of OGR1 gene in the biological activities of ovarian cancer, we studied the OGR1 effects on ovarian cancer cells, HEY cells.Methods OGR1 gene was transfected into HEY cell, in which endogenous expression is low. OGR1-overxepressed cells and vector-transfected cells were compared in different assays. Western blotting was employed to confirm the high expression level of OGR1. Cell proliferation was determined by MTT assay and cell doubling time assay. Cell migration assay （transwell assay） and cell adhesion assay were performed to determine the migration and adhesion potential of cells. Student＇s t test was employed for statistical analysis.Results Proliferation of OGR1-overexpressed cells was significantly reduced （P ＜0.01）; cell migration was significantly inhibited in the OGR1-transfected cells （P ＜0.01）; cell adhesion to extracellular matrix including fibronectin, vitronectin,collagen Ⅰ/Ⅳ was significantly increased （P ＜0.01）.Conclusions OGR1 expression in human ovarian cancer cells significantly inhibited the cell proliferation and migration,but significantly enhanced cell adhesion to the extracellular matrix. It indicated that OGR1 may be a tumor suppressor gene for ovarian cancer.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.2011.09.010