Role of co-expression of c-Myc, EZH2 and p27 in prognosis of prostate cancer patients after surgery

• Published in: Chinese medical journal 2013-01, Vol.126 (1), p.82-87
• Main Authors: Li, Ke, Chen, Ming-kun, Situ, Jie, Huang, Wen-tao, Su, Zu-lan, He, Dan, Gao, Xin
• Format: Article
• Language: English
• Subjects: c-Myc; Cyclin-Dependent Kinase Inhibitor p27 - analysis; Enhancer of Zeste Homolog 2 Protein; Humans; Immunohistochemistry; Male; Middle Aged; Neoplasm Recurrence, Local - epidemiology; Neoplasm Staging; p27; Polycomb Repressive Complex 2 - analysis; Prognosis; Prostatic Neoplasms - chemistry; Prostatic Neoplasms - mortality; Prostatic Neoplasms - pathology; Prostatic Neoplasms - surgery; Proto-Oncogene Proteins c-myc - analysis; 免疫组织化学; 共表达; 前列腺癌; 患者; 手术; 预后
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20122477

Background c-Myc, EZH2 and p27 were defined to modulate anti-tumoral effects and had ability in predicting prostate cancer value of prognosis is rarely. This study aimed to investigate the patients with intermediate-risk prostate cancer after surgery. the behavior of prostate cancer with pro-tumoral or progression, but the research of their co-expression prognostic value of combining tri-marker together in Methods Expression levels of c-Myc, EZH2 and p27 in 129 patients with intermediate-risk prostate cancer were assessed using immunohistochemistry in a semi-quantitative manner. The expression profiles of these three markers were analyzed and investigated for association with biochemical recurrence. Results In all, fifty of 129 cases experienced biochemical recurrence during a median follow-up time of 31 months （range, 6-60 months）. Of these relapse patients, one case without and 10 cases with any single positive marker were observed; 39 cases were detected with any two or all three positive markers （22 cases with any two and 17 cases with all three positive markers）. Survival analysis showed that patients with over-expression of c-Myc or EZH2, and lower expression of p27 manifested significantly higher biochemical recurrence rates. Subsequent multivariate analysis revealed that c-Myc, EZH2 and p27 expression statuses showed potential in predicting relapse, respectively. Notably, combining three markers together as a ＂composite index＂ （0 or 1, vs. 2 or 3 positive markers） provided powerful prognostic value （HR 6.57, 95% CI 3.02-14.31, P 〈0.001）. There was a significant difference between the patient subgroups with 0 or 1 and those with 2 or 3 positive markers expression statuses, and tri-marker composite index was an independent risk factor for predicting relapse in patients with intermediate-risk prostate cancer after surgery. Conclusion Composite index of c-Myc, EZH2, and p27 can be valued as powerful prognosis parameter for intermediate-risk prostate cancer patients after the surgery, and postoperative adjuvant therapy can be adopted accordingly.