H +-ATPase in the plasma membrane of Arabidopsis pollen cells is involved in extracellular calmodulin-promoted pollen germination

In this paper, the role of the plasma membrane (PM) H +-ATPase in extracellular calmodulin (CaM)-promoted pollen germination and in tube growth of Arabidopsis thaliana was investigated. Pollen germination, pollen tube growth rate, free cytosolic Ca 2+ concentration ([Ca 2+] cyt) and Ca 2+ channel ac...

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Bibliographic Details
Published in:Progress in natural science 2009, Vol.19 (9), p.1071-1078
Main Authors: Sun, Wending, Li, Sujuan, Xu, Junying, Liu, Ting, Shang, Zhonglin
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Extracellular calmodulin
H +-ATPase
Hyperpolarization-activated calcium channel
Pollen
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Summary:In this paper, the role of the plasma membrane (PM) H +-ATPase in extracellular calmodulin (CaM)-promoted pollen germination and in tube growth of Arabidopsis thaliana was investigated. Pollen germination, pollen tube growth rate, free cytosolic Ca 2+ concentration ([Ca 2+] cyt) and Ca 2+ channel activity in the PM of pollen cells were measured. In response to fusicoccin or CaM treatment, in vitro pollen germination and pollen tube growth rate, [Ca 2+] cyt and activity of a hyperpolarization-activated Ca 2+-permeable channel increased. Sodium vanadate inhibited the promotion of these parameters by extracellular CaM. The results suggest that H +-ATPase may be involved in extracellular CaM-regulated pollen germination and in tube growth by modulation of the hyperpolarization-activated Ca 2+ channel in the PM of A. thaliana pollen cells.
ISSN:1002-0071
DOI:10.1016/j.pnsc.2009.02.002