Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus (Fisch.) Bunge

A new water‐soluble hetero‐polysaccharide, APSID3, was obtained from a hot‐water extract of the roots of Astragalus membranaceus (Fisch.) Bunge by DEAE‐Sepharose Fast Flow and Sephacryl S‐300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 × 105Da. Based on a sugar compositio...

Full description

Saved in:
Bibliographic Details
Published in:Journal of integrative plant biology 2006-11, Vol.48 (11), p.1379-1384
Main Authors: Wang, Shun-Chun, Shan, Jun-Jie, Wang, Zheng-Tao, Hu, Zhi-Bi
Format: Article
Language:English
Subjects:
Astragalus membranaceus
hetero-polysaccharide
isolation and purification
structural analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new water‐soluble hetero‐polysaccharide, APSID3, was obtained from a hot‐water extract of the roots of Astragalus membranaceus (Fisch.) Bunge by DEAE‐Sepharose Fast Flow and Sephacryl S‐300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 × 105Da. Based on a sugar composition analysis, methylation analysis, partial hydrolysis and 13C nuclear magnetic resonance experimentation, it was concluded that the minimal repeat unit of APSID3 was composed of one terminal arabinose, one 1,5‐linked arabinose, one 1,3‐linked rhamnose, one 1,3,4‐linked rhamnose, five 1,4‐linked methyl galacturonates and six 1,4‐linked methyl glucuronates. (Managing editor: Wei Wang)
ISSN:1672-9072
1744-7909
DOI:10.1111/j.1744-7909.2006.00325.x