In vitro Transient Expression System of Latex C-serum was used for Analysis of Hevein Promoter in Response to Abscisic Acid in Hevea brasiliensis

• Published in: Journal of integrative plant biology 2008-03, Vol.50 (3), p.338-344
• Main Authors: Fei, Xiao-Wen, Deng, Xiao-Dong
• Format: Article
• Language: English
• Subjects: abscisic acid; Abscisic Acid - pharmacology; Antimicrobial Cationic Peptides - genetics; Base Pairing - drug effects; Centrifugation; Gene Expression Regulation, Plant - drug effects; Glucuronidase - metabolism; gus (beta-glucuronidase) gene; Hevea - drug effects; Hevea - genetics; hevein promoter; Latex - metabolism; Plant Leaves - cytology; Plant Leaves - drug effects; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Lectins - genetics; Promoter Regions, Genetic - genetics; rubber tree (Hevea brasiliensis); Sequence Deletion
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2007.00628.x

Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1 241-bp fragment of a 5' upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5' end nested deletion method in the present study, fused with a uidA (gus) gene to produce a series of tested constructs, which were transferred into C-serum of latex and the Gus activities were detected. Results showed that the fragment from -749 to -292 was sufficient for expression of gus gene in latex, and the fragment from -292 to -168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus-specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from -749 to -292 was laticiferous-specific.