Isolation and Characterization of a C-repeat Binding Transcription Factor from Maize

C-repeat binding proteins (CBFs) are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature- or dehydration-responsive cis-elements,...

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Bibliographic Details
Published in:Journal of integrative plant biology 2008-08, Vol.50 (8), p.965-974
Main Authors: Wang, Lei, Luo, Yanzhong, Zhang, Lan, Zhao, Jun, Hu, Zhiqiu, Fan, Yunliu, Zhang, Chunyi
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis
Base Sequence
C-repeat
DNA, Complementary - genetics
DNA-binding
Gene Expression Regulation, Plant
Genome, Plant
Models, Molecular
Molecular Sequence Data
Phylogeny
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - isolation & purification
Plant Proteins - metabolism
Protein Binding
Protein Structure, Tertiary
Repetitive Sequences, Nucleic Acid
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae - cytology
Sequence Alignment
transcription factor
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - isolation & purification
Transcription Factors - metabolism
Transformation, Genetic
Zea mays
Zea mays - genetics
Zea mays - metabolism
ZmCBF3
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Summary:C-repeat binding proteins (CBFs) are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature- or dehydration-responsive cis-elements, thus conferring plants cold acclimation. In the present study, we isolated a C-repeat binding transcription factor from maize using the yeast one-hybrid system with the C-repeat motif from the promoter of the Arabidopsis COR15a gene as bait. The isolated transcription factor is highly similar to the Arabidopsis CBF3 in their predicted amino acid sequences, and is therefore designated ZmCBF3. Point mutation analyses of the ZmCBF3-binding cis-element revealed (A/G)(C/T)CGAC as the core binding sequence. Expression analyses showed that ZmCBF3 was upregulated by both abscisic acid and low temperature, and was actively expressed during embryogenesis, suggesting that ZmCBF3 plays a role in stress response in maize.
ISSN:1672-9072
1744-7909
DOI:10.1111/j.1744-7909.2008.00683.x