Regulation of OsSPX1 and OsSPX3 on Expression of OsSPX domain Genes and Pi-starvation Signaling in Rice

• Published in: Journal of integrative plant biology 2009-07, Vol.51 (7), p.663-674
• Main Authors: Wang, Zhiye, Hu, Han, Huang, Hongjie, Duan, Ke, Wu, Zhongchang, Wu, Ping
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Down-Regulation - genetics; expression regulation; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; MicroRNAs - metabolism; Models, Biological; Molecular Sequence Data; Oryza - cytology; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Phosphates - deficiency; Phosphates - metabolism; Phylogeny; Pi-signaling; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Transport; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Signal Transduction; SPX domain gene; Subcellular Fractions - metabolism; Transcription, Genetic
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2009.00834.x

The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N‐terminal, designated as OsSPX1‐6. Here we report the diverse expression patterns of the OsSPX genes in different tissues and their responses to Pi‐starvation. Among them, five genes, OsSPX1, 2, 3, 5 and 6 are responsive to Pi‐starvation in shoots and/or in roots. The subcellular localization analysis indicates that OsSPX1 and OsSPX2 is exclusively located in nucleus, OsSPX3 in the cytoplasm, and OsSPX4 is a membrane localization protein. OsSPX1 regulates OsSPX2, 3 and 5 at the transcription level and is positively involved in the responses of the genes to Pi‐starvation. Overexpression of OsSPX3 downregulates OsSPX5 in shoots under Pi‐sufficiency. OsSPX3 negatively regulates the PSI (Pi‐starvation induced) gene, OsIPS1 and is involved in the responses of miR399 and OsPHO2 to Pi‐starvation. Our results suggest that OsSPX1 may be a regulator involved in the transcriptions of OsSPX2, 3 and 5. OsSPX3 plays a role in OsIPS1/miR399 mediated long distance regulation on OsPHO2. Our results also indicate that OsSPX3 is involved in plant tolerance to Pi‐starvation stress.