Different Responses of Plant Growth and Antioxidant System to the Combination of Cadmium and Heat Stress in Transgenic and Non-transgenic Rice

• Published in: Journal of integrative plant biology 2009-10, Vol.51 (10), p.942-950
• Main Authors: Zhao, Feng-Yun, Liu, Wen, Zhang, Shi-Yong
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; ascorbate-glutathione cycle; Ascorbic Acid - metabolism; Cadmium - toxicity; catalase; Catalase - metabolism; combined cadmium and heat stress; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; Glutathione - metabolism; Glutathione Transferase - metabolism; glutathione-S-transferase; Hot Temperature; Oryza - drug effects; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Oxidation-Reduction - drug effects; Plants, Genetically Modified - drug effects; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - metabolism; Stress, Physiological; Superoxide Dismutase - metabolism; transgenic rice
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2009.00865.x

A comparative study of just cadmium (Cd) or heat and their combination treatments on some physiological parameters and the antioxidant systems in transgenic rice (Oryza sativa L. cv. Zhonghua No.11) carrying glutathione-S-transferase (GST, EC. 2.5.1.18) and catalase1 (CAT1, EC. 1.11.1.6) and non-transgenics was conducted. The results revealed improved resistance in the transgenics to Cd and the combined Cd and heat stress than non-transgenics. Data showed that the activities of CAT, GST, superoxide dismutase (EC.1.15.1.1) and all components of the ascorbate-glutathione cycle measured in the stressed transgenics shoots are significantly different from those of non-transgenics. Results indicated that co-expression of GST and CAT1 had an important effect on the antioxidant system, in particular, the whole ascorbate-glutathione cycle. The less oxidative damage induced by Cd and the stress combination in the transgenics resulted not only from the GST and CAT1 transgene but also from the coordination of the whole ascorbate-glutathione cycle.