Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed tran...

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Published in:Journal of integrative plant biology 2011-03, Vol.53 (3), p.193-211
Main Authors: Yang, Jun, An, Dong, Zhang, Peng
Format: Article
Language:English
Subjects:
Gluconeogenesis - genetics
Gluconeogenesis - physiology
Glycolysis - genetics
Glycolysis - physiology
Manihot - enzymology
Manihot - genetics
Manihot - metabolism
Plant Proteins - metabolism
Plant Roots - enzymology
Plant Roots - genetics
Plant Roots - metabolism
Polymerase Chain Reaction
差异表达基因
活动过程
焦磷酸化酶
糖酵解
逆转录聚合酶链反应
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An, Dong
Zhang, Peng
description Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription fac- tors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. Tlhese studies should facilitate our understanding of the storage root formation and cassava improvement.
doi_str_mv 10.1111/j.1744-7909.2010.01018.x
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To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription fac- tors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. 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subjects Gluconeogenesis - genetics
Gluconeogenesis - physiology
Glycolysis - genetics
Glycolysis - physiology
Manihot - enzymology
Manihot - genetics
Manihot - metabolism
Plant Proteins - metabolism
Plant Roots - enzymology
Plant Roots - genetics
Plant Roots - metabolism
Polymerase Chain Reaction
差异表达基因
活动过程
焦磷酸化酶
糖酵解
逆转录聚合酶链反应
title Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis
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