Overexpression of INCREASED CAMBIAL ACTIVITY, a putative methyltransferase, increases cambial activity and plant growth

Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY （ICA）, a gene encoding a putative pectin methyltransferase, which could fu...

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Published in:Journal of integrative plant biology 2016-11, Vol.58 (11), p.874-889
Main Authors: Kim, Hyunsook, Kojima, Mikiko, Choi, Daeseok, Park, Soyoung, Matsui, Minami, Sakakibara, Hitoshi, Hwang, Ildoo
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cambial activity
Cambium - genetics
Cambium - growth & development
cytokinin
Cytokinins - metabolism
Gene Expression
gibberellin
Gibberellins - metabolism
methanol
methyltransferase
Methyltransferases - genetics
Methyltransferases - metabolism
pectin
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
shoot growth
分生组织
基因编码
形成层活动
径向生长
果胶甲基酯酶
植物生长
甲基转移酶
细胞活性
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Summary:Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY （ICA）, a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S：＇1CA, showed accelerated stern elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins （CKs） and gibberellins （GAs）. Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 355：：ICA, which might contribute to an increase of methanol emission as a byproduct in 35S：ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.12486