BRITTLE PLANT1 is required for normal cell wall composition and mechanical strength in rice

A series of nucleotide sugar interconversion enzymes (NSEs) generate the activated sugar donors required for biosynthesis of cell wall matrix polysaccharides and glycoproteins. UDP‐glucose 4‐epimerases (UGEs) are NSEs that function in the interconversion of UDP‐glucose (UDP‐Glc) and UDP‐galactose (U...

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Bibliographic Details
Published in:Journal of integrative plant biology 2021-05, Vol.63 (5), p.865-877
Main Authors: Zhang, Yuanyan, Wang, Yihua, Wang, Chunming, Rautengarten, Carsten, Duan, Erchao, Zhu, Jianping, Zhu, Xiaopin, Lei, Jie, Peng, Chao, Wang, Yunlong, Teng, Xuan, Tian, Yunlu, Liu, Xi, Heazlewood, Joshua L., Wu, Aimin, Wan, Jianmin
Format: Article
Language:English
Subjects:
Arabinogalactan
arabinogalactan proteins
Biosynthesis
Brittleness
Cell walls
Cellulose
Cloning
Composition
Epimerase
Galactose
Glucose
Glycoproteins
Mechanical properties
Monosaccharides
Mutants
Nucleotides
Oryza sativa
Polysaccharides
Proteins
Proteoglycans
Redundancy
Rhamnogalacturonan
rhamnogalacturonan I
Rice
Saccharides
Sugar
UDP‐galactose/glucose 4‐epimerase
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Summary:A series of nucleotide sugar interconversion enzymes (NSEs) generate the activated sugar donors required for biosynthesis of cell wall matrix polysaccharides and glycoproteins. UDP‐glucose 4‐epimerases (UGEs) are NSEs that function in the interconversion of UDP‐glucose (UDP‐Glc) and UDP‐galactose (UDP‐Gal). The roles of UDP‐glucose 4‐epimerases in monocots remain unclear due to redundancy in the pathways. Here, we report a brittle plant (bp1) rice mutant that exhibits brittle leaves and culms at all growth stages. The mutant culms had reduced levels of rhamnogalacturonan I, homogalacturonan, and arabinogalactan proteins. Moreover, the mutant had altered contents of uronic acids, neutral noncellulosic monosaccharides, and cellulose. Map‐based cloning demonstrated that OsBP1 encodes a UDP‐glucose 4‐epimerase (OsUGE2), a cytosolic protein. We also show that BP1 can form homo‐ and hetero‐protein complexes with other UGE family members and with UDP‐galactose transporters 2 (OsUGT2) and 3 (OsUGT3), which may facilitate the channeling of Gal to polysaccharides and proteoglycans. Our results demonstrate that BP1 participates in regulating the sugar composition and structure of rice cell walls. The composition and physical properties of cell wall materials are tightly regulated to support plant growth and development. UDP‐galactose/glucose 4‐epimerase is involved in regulating cell wall composition and thus modulating cell wall mechanical strength in rice.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.13050