Novel Wx alleles generated by base editing for improvement of rice grain quality

Amylose content (AC), which is regulated by the Waxy (Wx) gene, is a major indicator of eating and cooking quality (ECQ) in rice (Oryza sativa). Thus far, only a limited number of mutations in the N‐terminal domain of Wx were found to have a major impact on the AC of rice grains and no mutations wit...

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Bibliographic Details
Published in:Journal of integrative plant biology 2021-09, Vol.63 (9), p.1632-1638
Main Authors: Huang, Xiaorui, Su, Fei, Huang, Sheng, Mei, Fating, Niu, Xiaomu, Ma, Changle, Zhang, Hui, Zhu, Xiaoguo, Zhu, Jian‐Kang, Zhang, Jinshan
Format: Article
Language:English
Subjects:
Adenine
adenine base editing
Alleles
Amino acid sequence
Amino acids
Amylose
Amylose - genetics
Amylose - ultrastructure
amylose content (AC)
Cooking
Cytosine
cytosine base editing
eating and cooking quality (ECQ)
Edible Grain - chemistry
Edible Grain - genetics
Gene Editing
Grain
grain appearance
Mutation
Nucleotides
Oryza - chemistry
Oryza - genetics
Oryza sativa
Plant breeding
Plant Proteins - genetics
Progeny
Rice
Starch Synthase - genetics
Transgenes
Waxy gene
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Summary:Amylose content (AC), which is regulated by the Waxy (Wx) gene, is a major indicator of eating and cooking quality (ECQ) in rice (Oryza sativa). Thus far, only a limited number of mutations in the N‐terminal domain of Wx were found to have a major impact on the AC of rice grains and no mutations with such effects were reported for other regions of the Wx protein. Here, nucleotide substitutions in the middle region of Wx were generated by adenine and cytosine base editors. The nucleotide substitutions led to changes in 15 amino acid residues of Wx, and a series of novel Wx alleles with ACs of 0.3%–29.43% (wild type with AC of 19.87%) were obtained. Importantly, the waxyabe2 allele showed a “soft rice” AC, improved ECQ, favorable appearance, and no undesirable agronomic traits. The transgenes were removed from the waxyabe2 progeny, generating a promising breeding material for improving rice grain quality. The waxyabe2 allele in the middle region of the Wx gene was generated by adenine and cytosine base editors. The mutants showed a “soft rice” amylose content, improved eating and cooking quality, favorable appearance, and no undesirable agronomic traits.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.13098