The role of senescence‐associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar

Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from...

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Published in:Journal of integrative plant biology 2022-01, Vol.64 (1), p.73-86
Main Authors: He, Hui, Song, Xue‐Qin, Jiang, Cheng, Liu, Ying‐Li, Wang, Dian, Wen, Shuang‐Shuang, Chai, Guo‐Hua, Zhao, Shu‐Tang, Lu, Meng‐Zhu
Format: Article
Language:English
Subjects:
cambium
Cell differentiation
Cell division
Cell proliferation
Cell walls
Diameters
Differentiation
Downstream effects
Hardwoods
Homology
PagSAG101a
Phenotypes
Plant cells
Poplar
Populus
secondary cell wall
Senescence
Stems
Thickening
Transcriptomes
Transgenic plants
Wall deposition
Woody plants
Xylem
xylem formation
Zinc finger proteins
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Summary:Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba × Populus glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non‐transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock‐out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagSAG101a, downstream of PagC3H17, functions in wood development. Poplar Senescence‐associated gene101a (PagSAG101a), an ortholog of Arabidopsis AtSAG101, plays an important role in regulating secondary xylem development. In addition, the tandem CCCH zinc finger protein PagC3H17 directly regulates PagSAG101a expression, providing insight into the mechanisms regulating xylem differentiation in poplar.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.13195