3′‐(2,4‐Dinitrobenzenesulfonyl)‐2′,7′‐dimethylfluorescein as a Fluorescent Probe for Selenols

Simply the BESThio: The fluorescein derivative BESThio (see scheme) operates as a thiol probe at pH 7.4 and functions as a selenol probe at pH 5.8, and can thus be selectively applied to the rapid quantification of selenocysteine and the determination of the selenocysteine content in selenoproteins...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2006-03, Vol.45 (11), p.1810-1813
Main Authors: Maeda, Hatsuo, Katayama, Kohei, Matsuno, Hiromi, Uno, Tadayuki
Format: Article
Language:English
Subjects:
analytical methods
fluorescent probes
proteins
selenium
Online Access:Get full text
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Summary:Simply the BESThio: The fluorescein derivative BESThio (see scheme) operates as a thiol probe at pH 7.4 and functions as a selenol probe at pH 5.8, and can thus be selectively applied to the rapid quantification of selenocysteine and the determination of the selenocysteine content in selenoproteins such as glutathione peroxidase and thioredoxin reductase with high sensitivity.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200504299