Turkish population data on the factor XIII Val34Leu,glycoprotein (GP)Ibα Kozak and P-selectin glycoprotein ligand 1 (PSGL-1) loci

• Published in: Cell biochemistry and function 2005-01, Vol.23 (1), p.55-58
• Main Authors: Hancer, Veysel Sabri, Diz-Kucukkaya, Reyhan, Nalcaci, Meliha
• Format: Article
• Language: English
• Subjects: FXIII Val34Leu; gene polymorphism; GPIbα Kozak; PSGL-1; Turkish population
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1150

We determined the allele and genotype frequencies of three PCR‐based gene polymorphisms factor XIII (FXIII) Val34Leu, glycoprotein (GP) Ibα Kozak and P‐selectin glycoprotein ligand 1 (PSGL‐1) in the Turkish population (n=126 for FXIII Val34Leu, n=110 for GPIbα Kozak and n=203 for PSGL‐1). To detect these polymorphisms, DNA was extracted from venous blood. Genomic DNA samples were replicated and analysed by a polymerase chain reaction (PCR) method. PCR products were digested by restriction endonuclease enzymes for FXIII Val34Leu and GPIbα Kozak. PSGL‐1 was analysed by variable number of tandem repeats (VNTR). Allele frequencies of V (Val) and L (Leu) were found to be 0.805 and 0.195 respectively for the FXIII Val34Leu polymorphism. No significant difference was observed between French and Turkish populations for FXIII Val34Leu. Allele frequencies of T and C were calculated to be 0.873 and 0.127 for the GPIbα Kozak polymorphism and no significant difference was found between Turkish and French populations. In contrast, the difference between Turkish and Japanese populations was statistically significant ‐p