Study on the sorption properties of α1-acid glycoprotein (AGP)-based stationary phase modified by organic solvents

An α1‐acid glycoprotein, immobilized on silica (Chiral‐AGP) is one of the most widely used chiral stationary phases for the enantiomeric separation of a wide variety of chiral drugs with several applications in the biological and clinical field. The aim of this work was to study the sorption propert...

Full description

Saved in:
Bibliographic Details
Published in:Chirality (New York, N.Y.) N.Y.), 2003, Vol.15 (5), p.377-381
Main Authors: Gyimesi-Forrás, Krisztina, Szász, György, Báthory, Gábor, Mészáros, György, Gergely, András
Format: Article
Language:English
Subjects:
Chiral-AGP
enantioselectivity
fluorescence quenching
organic modifiers
trichloroethanol
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An α1‐acid glycoprotein, immobilized on silica (Chiral‐AGP) is one of the most widely used chiral stationary phases for the enantiomeric separation of a wide variety of chiral drugs with several applications in the biological and clinical field. The aim of this work was to study the sorption properties of the AGP‐based stationary phase, which may have crucial importance for enantioselectivity. New binding data to the mechanism of the chromatographic separation are presented. The sorption of both organic solvents, i.e., acetonitrile and dioxane, shows remarkable pH dependency. A fluorescence quenching study was carried out to elucidate structural changes of AGP in the presence of acetonitrile using 2,2,2‐trichloroethanol as fluorescence quencher. Chirality 15:377–381, 2003. © 2003 Wiley‐Liss, Inc.
ISSN:0899-0042
1520-636X
DOI:10.1002/chir.10213