Binding Constant of VIVO to Transferrin

The interactions of bis‐VIVO‐1,2‐dimethyl‐3‐hydroxy‐4(1H)‐pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed,...

Full description

Saved in:
Bibliographic Details
Published in:European Journal of Inorganic Chemistry 2006, Vol.2006 (18), p.3607-3613
Main Authors: Kiss, Tamás, Jakusch, Tamás, Bouhsina, Saâd, Sakurai, Hiromu, Enyedy, Éva Anna
Format: Review
Language:English
Subjects:
EPR spectroscopy
Stability constants
UV spectroscopy
VIVO-Transferrin complexes
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The interactions of bis‐VIVO‐1,2‐dimethyl‐3‐hydroxy‐4(1H)‐pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of VIVO to apoTF. A value of log K = 14.3 ± 0.6 was obtained for the binding of the first VIVO to apoTF. In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between VIVO–apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between VIVO–apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
ISSN:1434-1948
1099-0682
DOI:10.1002/ejic.200600314