Role of AgNPs in the enhancement of seed germination and its effect on plumule and radicle length of Pennisetum glaucum

• Published in: IET nanobiotechnology 2018-10, Vol.12 (7), p.922-926
• Main Authors: Sable, Shital Vishnu, Ranade, Suvidya, Joshi, Satyawati
• Format: Article
• Language: English
• Subjects: agriculture; analysis of variance; atomic emission spectroscopy; Bacillus - metabolism; Bacillus subtilis spizizenni; Bajara seeds; electron volt energy 3.0 keV; energy dispersive X‐ray spectroscopy; Fourier transform infrared spectra; Fourier transform infrared spectroscopy; Germination - drug effects; Green Chemistry Technology; inductively coupled plasma atomic emission spectroscopy; Metal Nanoparticles - chemistry; nanofabrication; nanoparticles; optical absorption spectrum; Pennisetum - drug effects; Pennisetum - growth & development; Pennisetum glaucum; Plant Shoots - drug effects; plumule length; radicle length; Research Article; scanning electron microscopy; Seeds - drug effects; silver; Silver - chemistry; Silver - pharmacology; silver nanoparticles; statistical analysis; time 3.0 d; transmission electron microscopy; Tukey's test; ultraviolet spectra; ultraviolet–visible absorption spectroscopy; visible spectra; X‐ray chemical analysis; X‐ray diffraction
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2017.0304

The authors have investigated beneficial effects of 1 mM of silver nanoparticles (AgNPs) on agriculturally important plant Pennisetum glaucum (Bajara). The extracellular AgNPs were synthesised using Bacillus subtilis spizizenni and characterised using ultraviolet–visible absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). Optical absorption spectrum showed characteristic peak of AgNPs at 423 nm. FT-IR analysis of AgNPs showed peak at 3435 cm−1, which indicates the presence of N–H group (primary, secondary amines and amides) on the surface of AgNPs. TEM studies indicate that synthesised AgNPs have average size of ∼2 nm. Energy dispersive X-ray spectroscopy showed strong signal of Ag at 3 keV. Treatment of 1 mM AgNPs to the bajara seeds was found to be sufficient for excellent germination of seeds within 3 days. There was also significant increase in radicle and plumule length as compared with control bajara seeds according to statistical analysis by one-way analysis of variance, followed by Tukey's test. The percentage of AgNPs detected in root samples was 0.003% (by inductively coupled plasma atomic emission spectroscopy), which is negligible. There is still need to study the bioavailability and the type of interaction of AgNPs with plants, necessary for application in agriculture.