Efficacy of mycosynthesised AgNPs from Earliella scabrosa as an in vitro antibacterial and wound healing agent

• Published in: IET nanobiotechnology 2019-05, Vol.13 (3), p.339-344
• Main Authors: Kithiyon, Mosachristas, Pannerselvam, Balashanmugam, Balasaubramaniyam Madhukumar, Sandilya Sharma, Sridharan, Jagadeeswari, Alagumuthu, Tamil Selvi
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; antibacterial activity; aqueous extract; Bacillus subtilis; biomedical materials; biomolecules; chemical properties; crystalline nature; Earliella scabrosa; EDX; energy dispersive X‐ray analysis; Escherichia coli; FESEM; field emission scanning electron microscopy; Fourier transform infrared spectra; Fourier transform infrared spectroscopy; FTIR spectroscopy; Gram‐negative bacteria; Gram‐positive bacteria; healing efficacy; high‐resolution transmission electron microscopy; in vitro antibacterial efficacy; In Vitro Techniques; macrofungus; Metal Nanoparticles - chemistry; microorganisms; molecular biophysics; mycosynthesised AgNPs; nanofabrication; nanomedicine; nanoparticles; particle size; pathogens; physical properties; Polyporaceae - chemistry; Pseudomonas aeruginosa; reduction (chemical); Research Article; silver; Silver - chemistry; silver nanoparticles; spherical‐shaped AgNPs; Staphylococcus aureus; surface plasmon resonance; surface plasmon resonance peak; therapeutical agent; transmission electron microscopy; wound closure; Wound Healing - drug effects; wound healing agent; wounds; XRD; X‐ray chemical analysis; X‐ray diffraction
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2018.5237

The silver nanoparticles (AgNPs) with their unique chemical and physical properties are proving as a new therapeutical agent. In the present study, the AgNPs synthesised from an aqueous extract of a macrofungus, Earliella scabrosa, were characterised by field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDX), high-resolution transmission electron microscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and further evaluate for its in vitro antibacterial and wound healing efficacy. The mycosynthesised AgNPs exhibited the surface plasmon resonance peak at 410 nm with good stability over a period of a month. The FESEM and EDX analyses revealed the spherical-shaped AgNPs of an average size of 20 nm and the presence of elemental Ag, respectively. The XRD pattern showed the crystalline nature of AgNPs. The FTIR spectra confirmed the conversion of Ag+ ions to AgNPs due to reduction by biomolecules of macrofungus extract. The mycosynthesised AgNPs showed effective antibacterial activity against two Gram-positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The pathogens were highly sensitive to AgNPs, whereas less sensitive to AgNO3. The mycosynthesised AgNPs showed significant wound healing potential with 68.58% of wound closure.