Facile synthesis of multi-walled carbon nanotube via folic acid grafted nanoparticle for precise delivery of doxorubicin

• Published in: IET nanobiotechnology 2019-09, Vol.13 (7), p.688-696
• Main Authors: Uttekar, Pravin S, Lakade, Sameer H, Beldar, Vijay K, Harde, Minal T
• Format: Article
• Language: English
• Subjects: 1 H NMR; acid functionalised MWCNT; anticancer drug doxorubicin; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; biocompatible targeted drug delivery; biological system; biomedical materials; cancer; cancer therapy; Cell Survival - drug effects; cellular biophysics; Coated Materials, Biocompatible - chemical synthesis; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - therapeutic use; composite material; cytotoxicity; differential scanning calorimetry; Doxorubicin - administration & dosage; Doxorubicin - pharmacokinetics; Drug Carriers - chemical synthesis; Drug Carriers - chemistry; Drug Compounding - methods; Drug Delivery Systems; drug entrapment efficiency; Drug Liberation; drugs; DSC; EDX; facile synthesis; FA‐conjugated MWCNT; FA‐EDA‐MWCNT‐DOX conjugate; folate‐conjugated MWCNTs; Folic Acid - chemistry; Folic Acid - therapeutic use; folic acid grafted nanoparticle; folic acid‐ethylene diamine; Fourier transform infrared spectra; FTIR spectra; human breast cancer MCF‐7 cells; Humans; in vitro anticancer activity; Materials Testing; MCF-7 Cells; MTT study; multiwalled carbon nanoplatform; multiwalled carbon nanotube; multi‐wall carbon nanotubes; nanocomposites; nanofabrication; nanomedicine; nanoparticles; Nanoparticles - chemistry; Nanoparticles - therapeutic use; Nanotubes, Carbon - chemistry; organic compounds; overexpressed folate receptor; precise delivery; proton magnetic resonance; Research Article; scanning electron microscopy; SEM; stability study; toxicology; XRD; X‐ray chemical analysis; π‐π stacking interaction
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2018.5421

The motive of work was to develop a multi-walled carbon nanoplatform through facile method for transportation of potential anticancer drug doxorubicin (DOX). Folic acid (FA)-ethylene diamine (EDA) anchored and acid functionalised MWCNTs were covalently grafted with DOX via π–π stacking interaction. The resultant composite was corroborated by 1H NMR, FTIR, XRD, EDX, SEM, and DSC study. The drug entrapment efficiency of FA-conjugated MWCNT was found high and stability study revealed its suitability in biological system. FA-EDA-MWCNTs-DOX conjugate demonstrated a significant in vitro anticancer activity on human breast cancer MCF-7 cells. MTT study revealed the lesser cytotoxicity of folate-conjugated MWCNTs. The obtained results demonstrated the targeting specificity of FA-conjugate via overexpressed folate receptor deemed greater scientific value to overcome multidrug protection during cancer therapy. The proposed strategy is a gentle contribution towards development of biocompatible targeted drug delivery and offers potential to address the current challenges in cancer therapy.