99mTc‐anti‐epidermal growth factor receptor nanobody for tumor imaging

Nanobodies are important biomolecules for tumor targeting. In this study, we synthesized and labeled anti‐epidermal growth factor receptor (EGFR) nanobody OA‐cb6 with 99mTc(CO)3+ and evaluated its characteristics for targeting the EGFR in the A431 human epidermal carcinoma cell line. Nanobody radiol...

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Published in:Chemical biology & drug design 2017-04, Vol.89 (4), p.498-504
Main Authors: Piramoon, Majid, Hosseinimehr, Seyed Jalal, Omidfar, Kobra, Noaparast, Zohreh, Abedi, Seyed Mohammad
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Language:English
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99mTc
EGFR
epidermal growth factor receptor
nanobody
tumor imaging
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container_end_page 504
container_issue 4
container_start_page 498
container_title Chemical biology & drug design
container_volume 89
creator Piramoon, Majid
Hosseinimehr, Seyed Jalal
Omidfar, Kobra
Noaparast, Zohreh
Abedi, Seyed Mohammad
description Nanobodies are important biomolecules for tumor targeting. In this study, we synthesized and labeled anti‐epidermal growth factor receptor (EGFR) nanobody OA‐cb6 with 99mTc(CO)3+ and evaluated its characteristics for targeting the EGFR in the A431 human epidermal carcinoma cell line. Nanobody radiolabeling was achieved with high yield and radiochemical purity, and the radioconjugate was stable. Biodistribution results in nude mice exhibited a favorable tumor‐to‐muscle ratio at 4‐hr postinjection, and tumor location was visualized at 4 hr after injection of radiolabeled nanobody. Our result showed that the OA‐cb6‐99mTc‐tricarbonyl radiolabeled nanobody is a promising radiolabeled biomolecule for tumor imaging in cancers with high EGFR overexpression. This study described the preparation and radiolabeling of anti‐EGFR nanobody, OA‐cb6. The nanobody was labeled using 99mTc‐tricarbonyl. Results showed that radioconjugate had fast blood clearance and a favorable tumor uptake in A431‐xenografted nude mice.
doi_str_mv 10.1111/cbdd.12871
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subjects 99mTc
EGFR
epidermal growth factor receptor
nanobody
tumor imaging
title 99mTc‐anti‐epidermal growth factor receptor nanobody for tumor imaging
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