Loading…
GALACTOSE TRANSPORT ACROSS RAT SMALL INTESTINE IN VIVO FOLLOWING DISTAL RESECTIONS OF VARYING EXTENTS
The kinetics of D-galactose absorption across rat small intestine in sham, 50 and 80% distal intestinal resected animals, have been studied under in vivo conditions. After 5 months post-resection the total galactose absorption was increased. The relationship between total absorption of substrate and...
Saved in:
Published in: | Experimental physiology 1986-07, Vol.71 (3), p.423-431 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Request full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The kinetics of D-galactose absorption across rat small intestine in sham, 50 and 80% distal intestinal resected animals,
have been studied under in vivo conditions. After 5 months post-resection the total galactose absorption was increased. The relationship between total absorption
of substrate and its concentration in the perfusate showed a non-saturable component and a saturable one that can be inhibited
by phloridzin, both in control and remnant intestine. The slope of the line that represents the non-saturable component is
greater in remnant duodenum and jejunum, indicating that the apparent mass-transfer coefficient, K' D , was increased by distal resection. Non-linear fitting of the saturable component data gave an apparent maximal galactose
transport rate ( J' max , nmol/cm2. min) of 134 ± 13 (sham), 132 ± 9 (50% resected), 139 ± 9 (80% resected) in duodenum and of 103 ± 11 (sham) and
106 ± 12 in jejunum. Apparent Michaelis constants ( K' m , mM) of 12·1 ± 3·2 (sham), 12·3 ± 2·2 (50% resected) and 13·3 ± 2·2 (80% resected) in duodenum and of 11·6 ± 3·7 (sham) and
12·8 ± 3.7 (50% resected) in jejunum were found. Correction of the kinetic constants for the unstirred water layer (u.w.l.)
effects showed that the differences between ârealâ K D 's of the experimental groups increased, whereas ârealâ K m 's did not significantly change. There were no significant differences in duodenal galactose absorption between 50 and 80%
resected rats. |
---|---|
ISSN: | 0958-0670 0144-8757 1469-445X |
DOI: | 10.1113/expphysiol.1986.sp003001 |