THE MODULATION OF FORCE IN ISOLATED RAT EGTATREATED ANOCOCCYGEUS MUSCLE BY PHOSPHATE, CYCLIC AMP AND NORADRENALINE

Saponin treatment is commonly used to permeabilize the surface membrane of muscle cells. Incubation of isolated rat anococcygeus for 4 h in a low Ca 2+ and low Mg 2+ solution resulted in the muscle responding to Ca 2+ , phosphate and cyclic AMP in a similar way to a saponin-treated muscle. However,...

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Bibliographic Details
Published in:Experimental physiology 1989-11, Vol.74 (6), p.943-945
Main Authors: Crichton, C. A., SMITH, G. L., Miller, D. J., McGrath, J. C.
Format: Article
Language:English
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Summary:Saponin treatment is commonly used to permeabilize the surface membrane of muscle cells. Incubation of isolated rat anococcygeus for 4 h in a low Ca 2+ and low Mg 2+ solution resulted in the muscle responding to Ca 2+ , phosphate and cyclic AMP in a similar way to a saponin-treated muscle. However, unlike saponin-treated preparations, the maximal calcium-activated force was stable for many hours and the addition of noradrenaline caused contracture. These properties would permit this preparation to be used to study aspects of receptor-mediated excitation-contraction coupling.
ISSN:0958-0670
0144-8757
1469-445X
DOI:10.1113/expphysiol.1989.sp003365