Abstract 18736: Human Adipose Tissue Inflammation Decreases CD271-Positive Adipose Derived Stem Cells and Deteriorates Therapeutic Angiogenesis of Cell Transplantation for Critical Limb Ischemia

• Published in: Circulation (New York, N.Y.) N.Y.), 2016-11, Vol.134 (Suppl_1 Suppl 1), p.A18736-A18736
• Main Authors: Inoue, Oto, Usui, Soichiro, Takashima, Shin-ichiro, Hamaoka, Takuto, Kitano, Teppei, Ootsuji, Hiroshi, Nomura, Ayano, Iino, Kenji, Kato, Takeshi, Murai, Hisayoshi, Furusho, Hiroshi, Takemura, Hirohumi, Kaneko, Shuichi, Takamura, Masayuki
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539

IntroductionAdipose derived stem cells (ADSCs) have been tested in cardiovascular diseases (CVD). However, CVD patients often accompany adipose tissue (AT) inflammation triggered by impaired angiogenesis. Therefore, ADSCs obtained from AT with inflammation might not be suitable for angiogenic cell therapy.HypothesisWe hypothesized that AT inflammation would decrease the specific subset of ADSCs and impair the angiogenic efficacy of ADSCs in a hindlimb ischemia model.Methods and ResultsSeventeen patients who underwent cardiac surgery were recruited. Subcutaneous AT was obtained and digested with collagenase to isolate stromal vascular fraction. Flowcytometric analysis was performed to determine cell subsets as follows; 1) CD45+CD14+CD206+ AT macrophages for pro-inflammatory (M1CD11c+) or alternative (M2CD11c-) polarization, 2) CD45-CD34+ ADSCs for several subsets previously reported to be therapeutic in other studies (e.g. VEGFR2, CD271). Among studied markers, the frequency of CD271+ /CD45-CD34+ (31.0±3.6%) was negatively correlated with the ratio of M1 to M2 macrophages (0.08±0.02; r=-0.51, p