Comparison of point detection and area detection for point-scanning structured illumination microscopy

Structured illumination microscopy (SIM) is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed. The system resolution is affected by two typical detection modes: Point detection and area detection. However, a systematic analys...

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Bibliographic Details
Published in:Journal of innovative optical health science 2023-07, Vol.16 (4)
Main Authors: Wu, Wenshuai, Chen, Jiajie, Wang, Meiting, Wang, Lei, Zheng, Xiaomin, Li, Jia, Qu, Junle, Gao, Bruce Zhi, Shao, Yonghong
Format: Article
Language:English
Subjects:
Biological properties
Biological samples
Illumination
Image resolution
Imaging techniques
Microscopy
Point spread functions
Regular Research Article
Second harmonic generation
structured illumination microscopy
Super-resolution
Theoretical analysis
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Summary:Structured illumination microscopy (SIM) is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed. The system resolution is affected by two typical detection modes: Point detection and area detection. However, a systematic analysis of the imaging performance of the different detection modes of the system has rarely been conducted. In this study, we compared laser point scanning point detection (PS-PD) and point scanning area detection (PS-AD) imaging in nonconfocal microscopy through theoretical analysis and simulated imaging. The results revealed that the imaging resolutions of PS-PD and PS-AD depend on excitation and emission point spread functions (PSFs), respectively. Especially, we combined the second harmonic generation (SHG) of point detection (P-SHG) and area detection (A-SHG) with SIM to realize a nonlinear SIM-imaging technique that improves the imaging resolution. Moreover, we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.
ISSN:1793-5458
1793-7205
DOI:10.1142/S1793545823500104