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In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy
Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to a...
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| Format: | Default Article |
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2015
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| Online Access: | https://hdl.handle.net/2134/9114506.v1 |
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| _version_ | 1823267752094203904 |
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| author | Gal Schkolnik Matthias Schmidt Marco Mazza Falk Harnisch Niculina Musat |
| author_facet | Gal Schkolnik Matthias Schmidt Marco Mazza Falk Harnisch Niculina Musat |
| author_sort | Gal Schkolnik (841706) |
| collection | Figshare |
| description | Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to anaerobically colonize an Ag/AgCl solid interface, S. oneidensis has precipitated silver nanoparticles (AgNp), thus providing the means for a surface enhanced confocal Raman microscopy (SECRaM) investigation of its biofilm. The result is the in-situ chemical mapping of the biofilm as it developed over time, where the distribution of cytochromes, reduced and oxidized flavins, polysaccharides and phosphate in the undisturbed biofilm is monitored. Utilizing AgNp bio-produced by the bacteria colonizing the Ag/ AgCl interface, we could perform SECRaM while avoiding the use of a patterned or roughened support or the introduction of noble metal salts and reducing agents. This new method will allow a spatially and temporally resolved chemical investigation not only of Shewanella biofilms at an insoluble electron acceptor, but also of other noble metal nanoparticle-precipitating bacteria in laboratory cultures or in complex microbial communities in their natural habitats. |
| format | Default Article |
| id | rr-article-9114506 |
| institution | Loughborough University |
| publishDate | 2015 |
| record_format | Figshare |
| spelling | rr-article-91145062015-12-28T00:00:00Z In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy Gal Schkolnik (841706) Matthias Schmidt (4628) Marco Mazza (5569439) Falk Harnisch (841708) Niculina Musat (841709) Biofilms Chemical Precipitation Cytochromes Flavins Metal Nanoparticles Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phosphates Polysaccharides, Bacterial Shewanella Silver Spectrum Analysis, Raman Surface Properties Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to anaerobically colonize an Ag/AgCl solid interface, S. oneidensis has precipitated silver nanoparticles (AgNp), thus providing the means for a surface enhanced confocal Raman microscopy (SECRaM) investigation of its biofilm. The result is the in-situ chemical mapping of the biofilm as it developed over time, where the distribution of cytochromes, reduced and oxidized flavins, polysaccharides and phosphate in the undisturbed biofilm is monitored. Utilizing AgNp bio-produced by the bacteria colonizing the Ag/ AgCl interface, we could perform SECRaM while avoiding the use of a patterned or roughened support or the introduction of noble metal salts and reducing agents. This new method will allow a spatially and temporally resolved chemical investigation not only of Shewanella biofilms at an insoluble electron acceptor, but also of other noble metal nanoparticle-precipitating bacteria in laboratory cultures or in complex microbial communities in their natural habitats. 2015-12-28T00:00:00Z Text Journal contribution 2134/9114506.v1 https://figshare.com/articles/journal_contribution/In_situ_analysis_of_a_silver_nanoparticle-precipitating_Shewanella_biofilm_by_surface_enhanced_confocal_Raman_microscopy/9114506 CC BY 4.0 |
| spellingShingle | Biofilms Chemical Precipitation Cytochromes Flavins Metal Nanoparticles Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phosphates Polysaccharides, Bacterial Shewanella Silver Spectrum Analysis, Raman Surface Properties Gal Schkolnik Matthias Schmidt Marco Mazza Falk Harnisch Niculina Musat In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title | In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title_full | In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title_fullStr | In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title_full_unstemmed | In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title_short | In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy |
| title_sort | in situ analysis of a silver nanoparticle-precipitating shewanella biofilm by surface enhanced confocal raman microscopy |
| topic | Biofilms Chemical Precipitation Cytochromes Flavins Metal Nanoparticles Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phosphates Polysaccharides, Bacterial Shewanella Silver Spectrum Analysis, Raman Surface Properties |
| url | https://hdl.handle.net/2134/9114506.v1 |